动物造模 z-bio 2021-11-30 503

　　Objective: To determine the growth, reproduction and blood physiological and biochemical indexes of SPF C1 Niemann-Pick disease mice (Npc1-/- mice), and to provide theoretical basic data for the study of NPC1 patients.

　　Methods: ①Choose 40 male and female Npc1-/-, Npc1+/- and Npc1+/+ mice aged 0~77 days, weigh them regularly and draw a growth curve; ②Npc1-/- mice are mated and reproduced by Npc1+/- mice Generate and count the reproduction data of Npc1+/- mice for 4 consecutive generations; ③Detect the blood physiological and biochemical indexes of 60-day-old Npc1-/- and Npc1+/+ mice.

　　Results: ① The body weight of Npc1-/- mice increased with the increase of age before 7 weeks, and decreased with the increase of age after 7 weeks, until they died at about 11 weeks. The body weight of Npc1+/+ and Npc1+/- mice gradually increased with age, and there was no significant difference between the two. After 4 weeks, males gained more weight than females significantly faster than female mice; ②Npc1+/- mice had no significant differences in the number of females and males and the number of positives in different generations of mating and delivery interval, litter number, number of weaned piglets, number of weaned piglets Sex (P>0.05), and the weaning rate of the second generation was significantly greater than that of the first generation (P<0.05); ③The average red blood cell hemoglobin content (MCH) and average excess blood in the blood physiological indicators of Npc1-/- and Npc1+/+ mice The difference in oxidase index (MPXI) was significant (P<0.05), and="" the="" other="" differences="" were="" not="" significant="" p="">0.05). The difference in biochemical indicators of urea (UREA) was extremely significant (P<0.01), while aspartate aminotransferase (AST), glucose (GLU), lactate dehydrogenase (LDH), potassium (K) and copper ( Cu) and other differences are significant (P<0.05), and="" the="" other="" differences="" are="" not="" significant="" p="">0.05).

　　Conclusion: ①The growth curves of Npc1-/-, Npc1+/- and Npc1+/+ mice are different due to different genotypes and genders; ②Npc1+/- mice have no significant difference in reproductive ability in different generations; ③Npc1-/-and There are significant differences in some blood physiological and biochemical indexes of Npc1+/+ mice.