动物造模 z-bio 2021-11-30 453

　　Objective: To investigate the changes of blood cell classification and T cell subsets in blood, bone marrow, and spleen of Shkbp1-deficient mice (Shkbp-1-/-).

　　Methods: Shkbp-1-/- transgenic mice were used to identify genotypes by PCR; automatic blood tester was used to determine the classification of blood cells; flow cytometry was used to test the blood and bone marrow of Shkbp-1-/- and control mice , And T lymphocyte subsets in the spleen.

　　Result: Blood test results: Neutrophils and eosinophils have a tendency to increase, and there are significant differences. There was no significant difference in lymphocytes. The flow cytometry results showed that the CD4+CD8+ double positive cells in the blood of the control group decreased, and the CD3+ and CD4+ in the bone marrow increased. However, in the spleen tissue, the CD3+, CD4+, CD8+, CD4+CD8+ double positive cells showed a downward trend.

　　Conclusion: Shkbp1 participates in the maturation and differentiation of blood cells and affects the number of immune cells. This study lays the foundation for exploring how Shkbp1 participates in the differentiation of blood cells.