动物造模,胚胎移植 z-bio 2021-11-30 1074

　　Purpose: To investigate the changes in the average body growth and blood biochemical characteristics of the C57BL/6J (B6) mice after cryo-thawed embryo transfer.

　　Method: The experiment is divided into 3 groups. Experimental group I: Frozen-thawed embryo transfer group, 2-cell embryos of B6 mice after in vitro fertilization were frozen by EFS method, resuscitated, and transplanted to the mice (30 male mice) born in the oviduct of the recipient (ICR mice) 20 female mice); experimental group II: the first generation of the offspring (26 male mice and 17 female mice) from the mature mice produced by freeze-thaw embryo transfer and natural mating; control group: normal breeding and natural mating Offspring (20 male and female mice each). The three groups of mice were raised to 16 weeks of age, and their body weights were measured regularly every week, and blood was collected at 16 weeks of age. The serum biochemical values of the mice in each group were measured and analyzed and compared.

　　Results: The birth weight and average weight of 1-16 weeks of the experimental group I female mice were significantly higher than those of the control group (P<0.01), and the weight of the experimental group II female mice was significantly higher than that of the control group from 12 weeks (P<0.01). ), while only part of the age of male mice is different from that of the control group, while the other ages are not significant; the serum biochemical values of the experimental group I and experimental group II mice are compared with the control group except for AST, TP, and Ca. Except for no change, the other items have been significantly or extremely significantly adjusted upwards or downwards.

　　Conclusion: Freezing has a certain effect on the average body growth and serum biochemical value of the mice obtained from embryo transfer and their natural reproduction generation.