动物造模 z-bio 2021-11-30 285

　　Objective: To study the difference in organ index and hypoxia-related gene expression between Potentilla and Bama miniature pigs.

　　Method: TaqMan probe real-time quantitative PCR (real-time quantitative PCR) technology was used to detect changes in the expression of hypoxia-responsive genes in the heart, liver, spleen, lung, and kidney of Potentilla and Bama miniature pigs.

　　Results: The kidney and spleen indexes of Potentilla pigs are lower than Bama mini-pigs, and the heart and lung indexes of Potentilla pigs are higher than those of Bama mini-pigs. The expression levels of Vascular endothelial growth factor (VEGF) and hypoxia inducible factor 1 (HIF-1α) in the lung and kidney of Juma pigs were significantly higher than those of ordinary pigs (P<0.05) Or P<0.01); Erythropoietin (EPO) is only higher in lungs than Bama mini-pigs (P<0.05).

　　Conclusion: The organ index of different species may be the result of the evolutionary adaptation of the species to the material environment, energy environment, external pathogens, energy metabolism requirements and other comprehensive factors. The genes related to hypoxia in Juma pigs are significantly higher than those of Bama mini-pigs, which reveals that Juma pigs have stronger plateau adaptability in terms of genes.