动物模型,皮肤过敏 z-bo 2020-08-10 733

　　(1) Reproduction method White guinea pigs weighing 200-250 g and 4 to 5 weeks old were sensitized on the 1st and 8th day respectively, and challenged on the 15th day. Model animals were injected intracutaneously with 5% trichloroethylene olive oil solution, the smearing induction concentration was 40%, and the challenge concentration was 20%. The skin reaction of the model animals was observed and recorded 24 hours after the challenge. The animals were then anesthetized and blood was collected. The skin was taken aseptically and fixed for routine tissue sectioning, stained and observed under a light microscope.

　　(2) Model features: The serum total IgG content of model animals is significantly increased, and most guinea pig skins can have erythema and edema. The histopathological observation results of the model show that the epidermal spinous cell layer of the model animals is thickened, and the capillaries in the dermis are dilated. Edema is visible, and inflammatory cells such as eosinophils and lymphocytes are infiltrated, accompanied by perivascular inflammation. The mechanism of trichloroethylene-induced allergic dermatitis is not yet clear. It may be that immune cells are proliferated and activated by trichloroethylene, and the inflammatory mediators released after entering the antigen invasion site cause pathological changes in the skin. The histopathological observation results of the allergic animal model replicated by this method are similar to the delayed type hypersensitivity of the animal induced by dinitrochlorobenzene, so it is speculated that the guinea pig skin allergy induced by trichloroethylene is a delayed type hypersensitivity reaction.

　　(3) Comparative Medicine Trichloroethylene is a volatile fat-soluble small molecule organic chemical. It is absorbed through the respiratory tract and skin through human occupational exposure, which can cause heart, liver, kidney and nervous system damage. Its pathogenesis is mainly manifested as skin allergic damage. It is speculated that allergic contact dermatitis caused by trichloroethylene or its metabolites is related to the body's abnormal immune response process, but the pathogenesis is still unclear. Since the immune system is a more sensitive target of toxic effects, the skin allergy animal model established by this method can be used to study the changes in the immune and pathological indicators of the animal body after trichloroethylene sensitization, for further study of human trichloroethylene The immunological mechanism and pathological diagnosis methods of allergic dermatitis provide animal experimental evidence.