动物造模,乙型脑炎 z-bio 2021-11-30 602

　　Purpose: To understand the characteristics of Japanese encephalitis virus strains in miniature pigs.

　　Methods: The pig brain tissue was processed, inoculated with BHK21 cells, virus culture, indirect immunofluorescence test, neutralization test, electron microscopy observation, and reverse transcription-polymerase chain reaction (RT-PCR) to amplify the E region of the newly isolated strain The nucleotide sequence of the segment and PrM segment was sequenced for genotype identification.

　　Results: The brain tissues of 25 pigs were inoculated with BHK21 cells, and 3 tissue treatment solutions could cause BHK21 cells to shrink, aggregate and other lesions; 3 tissues inoculated with BHK21 lesion cell droplets for immunofluorescence test, all produced strong green fluorescence response ; Neutralization experiment results showed that the neutralization titers of the three new isolates were all 1:64; spherical virus particles with a diameter of about 40 nm were visible under the electron microscope; RT-PCR product sequencing results were relatively homologous to the vaccine strain’s E-segment nucleotides The sex is 95%; the three new isolates are all GⅢ Japanese encephalitis virus.

　　Conclusion: Experiments show that there is Japanese encephalitis virus infection in the small pig farm, which is GⅢ Japanese encephalitis virus.