动物造模 z-bio 2021-12-02 254

　　Objective: To diagnose and treat Beagle dogs infected with Demodex mites and observe histopathology.

　　method: direct smear method for microscopic examination, routine paraffin section and HE staining followed by histopathological observation.

　　Results: (1) Clinical observation: red spots appeared in the skin of the limbs, eyes, lower abdomen and other parts of the affected dog, hair loss on the affected area, thick and wrinkled skin on the limbs; (2) Routine blood examination: basically normal; (3) Microscope Observation: A large number of small worms and eggs of Demodex canis were found; (4) Histopathological observation: A large number of Demodex canis and eggs were seen in the hair follicles. The sebaceous glands and sweat glands were normal in structure, and no mites were parasitic. A large number of eosinophils and neutrophils infiltrated around the hair follicles, and the formation of multifocal granulomas can be seen, and the granulomas are oval; (5) Treatment plan: treatment with combination drugs, enhanced environmental control, and significant curative effect.

　　Conclusion: Granuloma caused by Demodex canine can be classified as immune granuloma; small worms may not destroy the sebaceous glands after infection. Whether the sebaceous glands are infected with the worms may be related to the type of the worms or the course of the infection.