动物造模 z-bio 2021-12-06 307

　　Objective: To analyze the application value of whole blood IFN-γ release test in the detection of Mycobacterium macaque.

　　Method: First, determine the basic IFN-γ content of the tuberculin test (TST) positive and negative rhesus monkey serum samples. Then, with PBS as a control and 200 IU bovine-PPD as a stimulating antigen, they were co-cultured with TST-negative and positive rhesus heparin anticoagulant blood for about 24 hours. Plasma was collected and the IFN-γ content was determined. The diagnostic efficacy of the whole blood IFN-γ release test was explored by analyzing the changes of plasma IFN-γ content before and after antigen stimulation and the stimulation index.

　　Result: The serum basic IFN-γ content of TST-positive macaques was significantly higher than that of TST-negative macaques, but the dispersion was larger. Before and after PPD stimulation, the plasma IFN-γ content of TST-negative macaques did not change significantly, while the plasma IFN-γ content of TST-positive macaques was significantly increased (P<0.01). The stimulation index results showed that TST-positive rhesus monkeys were significantly higher than TST-negative monkeys (P<0.01). ROC curve analysis shows that plasma IFN-γ content and stimulation index can be used as evaluation indicators for whole blood IFN-γ release test.

　　Conclusion: The experimental results of this study based on a small sample size confirmed that the whole blood IFN-γ release test is a useful supplementary method for the rapid diagnosis of Mycobacterium rhesus.