动物造模 z-bio 2021-12-06 304

　　Objective: To establish a black-lined hamster model infected with Babesia, to clarify the changes in the biological characteristics of the black-lined hamster after infection, and to provide basic data for the detection and control of Babesia.

　　Method: intraperitoneal injection of blood containing Babesia to infect black-lined hamsters, collect 5 animals each time on the 0th, 2, 4, 6, 8, 10, 12, 14, 16, 23, 30, and 37 days of the infection Anticoagulant blood and whole blood, prepare blood smears, detect the reproduction of the insects by Giemsa staining; isolate the blood total DNA, use REAL-TIME PCR to detect the reproduction of Babesia in the host; use automatic physiology and biochemistry The detector measures blood physiological and biochemical indicators; the heart, liver, spleen, lung, kidney and other organs were collected after the animals were sacrificed, and the organ coefficients were measured by weighing; the IL-2 concentration in the serum of infected animals was detected by ELISA.

　　Result: On the 4th day after the infection, the number of Babesia in the black-lined hamster was the largest, and then the overall decline, and there was a short-term increase on the 12th day. The liver and spleen have the largest changes in the organ coefficients of infected animals. The coefficients of the heart, lungs and kidneys fluctuate slightly throughout the infection period and are all within the normal range. The blood cells of the infected animals fluctuated, reaching peak values twice on the 10th and 23rd days, among which the leukocyte changes were the most dramatic; the detected changes in blood biochemical indicators peaked on the 12th day. The IL-2 in the serum of infected animals reached a peak on the 10th day, and then continued to decline.

　　Conclusion: Black-lined hamsters infected with Babesia have typical tick-borne parasitic characteristics. The pathogen invades and reaches the peak of reproduction within a week, and the pathogen can be latent in the host for a long time. The host immune response reaches its peak in the second week, and the immune-related organs and blood cells have obvious stress responses. According to this, the diagnosis and prevention of Babesiosis can be carried out in a targeted manner.