动物造模 z-bio 2021-12-13 419

　　Objective: To investigate the interference of Helicobacter hepatica infection on the surface molecular morphology and immune response of mouse bone marrow-derived dendritic cells (DC).

　　Methods: SPF BALB/c male mice were fed with H. hepaticus (ATCC 51450), bone marrow DC were isolated and cultured in vitro 5 months after the last vaccination, and the granulocyte-monocyte colony stimulating factor (GM-CSF), Interleukin-4 (IL-4) stimulates the proliferation and differentiation of DCs. Flow cytometry analyzes the expression rates of DC cell surface molecules CD11c, CD40, CD80, and MHCⅡ. On this basis, the experimental group and the control group mice were artificially inoculated with the Newcastle disease virus (NDV) ZJ1 strain, and the NDV serum antibody titer was measured weekly to compare the difference in antibody production.

　　Result: The expression rate of MHC Ⅱ and CD40 molecules in the experimental group was higher than that in the control group. The NDV antibody level in the first week of the experimental group was slightly lower than that of the control group; in the 2nd to 5th week, the antibody level of the experimental group was higher than that of the control group, and the difference was significant; in the 6th week, the serum antibodies of the two groups of mice decreased Trend, the difference is not significant.

　　Conclusion: H. hepaticus infection promotes the maturation of mouse bone marrow DCs, can increase the expression levels of MHC Ⅱ and CD40, and can promote the production of anti-NDV antibody levels in BALB/c mice.