动物造模 z-bio 2021-12-13 313

　　OBJECTIVE: To establish an experimental system for mouse swimming endurance, to discover new indicators to evaluate the swimming endurance of mice, and to use this system to verify the effect of Rhodiola on the endurance of mice.

　　Methods: Computer video acquisition technology and image analysis technology were applied to the mouse swimming endurance experiment, and the mouse swimming information was obtained in real time. The extraction included exhaustion time, first sinking time, total sinking time, and first continuous sinking time. The multiple endurance evaluation indicators of, objectively and accurately analyze the mouse endurance data online; use normal mice and rhodiola-administered mice to conduct experimental research on the stability and reliability of the system.

　　Result: Computer recorded data and manual recorded data are highly correlated, r>0.95, the system is stable and reliable. The correlation coefficient between the first continuous sinking time and the exhaustion time is r=0.974, and the correlation coefficient between other new indicators and the exhaustion time is r<0.9. Compared with the blank group, the Rhodiola administration group had significantly longer time to first sinking and first continuous sinking.

　　Conclusion: The system is used to evaluate the indicators of mouse swimming endurance to be objective, accurate, and sensitive. It realizes the integration of video collection, information integration, data analysis, and statistical output, and finds new indicators that are highly related to exhaustion time-the first continuous download Sink time. The full set of systems are automated and highly intelligent, providing a reliable experimental system for the screening of anti-fatigue drugs.