Purpose: Through the Salmonella detection ability verification plan in laboratory animals, understand the ability of laboratory animal testing institutions to detect Salmonella, and improve the quality detection level of laboratory animals.
Method: In accordance with the proficiency testing program approved by CNAS, fecal samples of laboratory animals containing Salmonella and interfering bacteria were prepared by freeze-drying, and passed the stability and uniformity inspection as the proficiency testing sample. Random numbers are used and distributed to participating units via cold chain transportation, together with work instructions. Submit the inspection report and copy of the original record within the specified time limit. If the result is consistent with the sample pre-inspection result, it will be considered as a satisfactory result, and if the result is inconsistent or failing to submit the result, it will be considered as an unsatisfactory result.
Results: A total of 30 laboratories in 20 provinces and cities across the country participated in the Salmonella proficiency testing program, of which 28 laboratories received satisfactory results, accounting for 93.3% of the total participating institutions, and 2 laboratories were dissatisfied, accounting for 6.7%. There are 29 laboratories adopting the separation culture method, and 2 laboratories adopting the PCR method.
Conclusion: The laboratory animal quality inspection agency has a high ability to detect salmonella, and the implementation of the proficiency testing plan can reflect the laboratory's testing level.