动物造模 z-bio 2021-12-14 441

　　OBJECTIVE: To investigate the effects of environmental temperature on the body weight, brown adipose tissue (BAT) thermogenic activity and uncoupling protein 1 content of Chinese and Burmese tree shrews, and to provide a theoretical basis for the establishment of tree shrew obesity models.

　　Methods: 40 adult Chinese and Burmese tree shrews with similar weight were randomly divided into 5 groups (8 in each group): control group (0 d), kept at (25±1)℃, 12 L/12 D; And placed at (5 ± 1) ℃, 12 L/12 D conditions were acclimated 7, 14, 21 d and 28 d groups. After acclimatization, the animal's body weight, non-shivering thermogenesis (NST), brown adipose tissue weight, and uncoupling-catenin 1 (UCP1) content were measured.

　　Results: Compared with the control group (0 d), the body weight, NST, BAT weight and UCP1 content of Burmese tree shrews in the cold acclimation group were significantly increased, and the color of BAT was also significantly darker. After 28 days of cold acclimation, the body weight increased by 26.32% , NST increased by 20.65%, BAT weight increased by 53.85%, UCP1 content increased by 43%. UCP1 content is significantly positively correlated with BAT weight and NST.

　　Conclusion: The Chinese and Burmese tree shrews may induce BAT tissue proliferation and up-regulation of UCP1 expression through cold acclimation, thereby enhancing the thermogenesis activity of BAT to increase energy expenditure. It is speculated that BAT may be a target organ for the treatment of obesity through energetics.