动物造模,糖尿病足溃疡 z-bio 2021-12-31 619

　　Objective To explore the effects of salidroside (Sal) on the nuclear transcription factor E2 related factor 2 / Kelch-like epichlorohydrin related protein 1 (Nrf2 / Keap1) signaling pathway and wound healing in rats with diabetic foot ulcer (DFU).

　　Methods High-fat and high-sugar feed combined with intraperitoneal injection of streptozotocin (STZ) was used to establish a diabetic rat model, and the back of the foot was shaved and cut to the fascia to create an ulcer wound with an area of about 3 mm×7 mm. DFU rat models were randomly divided into DFU model group (DFU group), low Sal (Sal-L, 0.1 g / (kg d)), medium (Sal-M, 0.2 g / (kg d) )) high (Sal-H, 0.3 g / (kg d)) dose group, positive drug metformin group (MET group, 0.65 g / (kg The control group (NC group) received continuous gastric gavage for 2 weeks. The body weight and fasting blood glucose (FBG) levels of rats in each group were measured on the 7th and 14th days of treatment, the CD34 expression of the wound tissue was detected by immunohistochemistry, and the wound microvessel density (MVD) was calculated. SOD level; Western blot was used to detect the expression of Nrf2 and Keap1 protein in wound tissue.

　　Results There was no statistically significant comparison between the body weights of the rats in each group on the 0th day of treatment (P>0.05), and the rats in the DFU group, Sal-L group, Sal-M group, Sal-H group, and MET group were FBG in each group. The level is higher than the NC group (P<0.05); The 7th and 14th day of treatment, compared with the NC group, DFU group, Sal-L group, Sal-M group, Sal-H group, MET group rats Body weight, FBG level, MDA content, Keap1 protein expression were all increased (P<0.05), wound healing rate, CD34 positive cells, MVD, SOD activity, and Nrf2 protein expression were significantly reduced (P<0.05); Compared with the DFU group, the weight, FBG level, MDA content, and Keap1 protein expression of rats in the Sal-L group, Sal-M group, Sal-H group, and MET group were significantly reduced (P<0.05), and the wound healing rate was significantly reduced. , CD34 positive cells, MVD, SOD activity, and Nrf2 protein expression increased significantly (P<0. sal-l="" met="" p="">0. 05).

　　Conclusion Sal may increase the antioxidant capacity of DFU rats and promote wound healing by regulating the Nrf2/Keap1 signaling pathway.