动物造模 z-bio 2021-12-31 268

　　Objective To investigate the effect of oxidative stress on the proliferation, apoptosis, collagen synthesis and expression of inflammatory factors in uterine ligament fibroblasts and its possible mechanism.

　　Method The third generation rat uterine ligament fibroblasts with good growth conditions were divided into low oxidative stress group, high oxidative stress group, and control group. Choose 0.2 mmol/L and 0.8 mmol/L hydrogen peroxide to intervene uterine ligament fibroblasts for 4 hours to establish a low-level and high-level oxidative stress model. The control group did not perform any intervention. MTT method was used to detect cell proliferation ability, Annexin V-FITC/PI method was used to detect cell apoptosis, and Western blot method was used to detect the protein levels of type Ⅰ collagen, type Ⅲ collagen, inflammatory factors, and proteins related to signal pathways.

　　Results Compared with the control group and the low oxidative stress group, the cell proliferation ability of the high oxidative stress group decreased (P<0.05), the apoptotic rate increased (P <0.05), the synthesis of type Ⅰ collagen and type Ⅲ collagen Significantly reduced (P <0.05), and the protein expression levels of interleukin 1β, tumor necrosis factor alpha and interleukin 6 increased (P <0.05); compared with the control group and the low oxidative stress group, high The expression of p-ERK1/2 and p-Akt in the oxidative stress group was significantly reduced (P <0.05), while the expression of total protein ERK1/2 and Akt remained basically unchanged. There was no significant difference between the above indicators in the low oxidative stress group and the control group.

　　Conclusion The higher concentration of hydrogen peroxide in the oxidative stress microenvironment can inhibit the expression of ERK1/2 in the MAPK pathway and the expression of Akt in the PI3K-Akt pathway, causing the proliferation of uterine ligament fibroblasts, the decrease in collagen synthesis ability, and the apoptotic cells. Increased expression of inflammatory factors participates in the occurrence and development of pelvic organ prolapse.