动物造模,肺组织损伤 z-bio 2021-12-31 837

　　Objective To evaluate the effects of cobalt, chromium and molybdenum metal (trade name: Vitalin), zirconium dioxide porcelain, and polymethyl methacrylate resin three kinds of dental grinding dust on lung tissue damage in rats.

　　Methods Scanning electron microscopy and laser particle size analyzer were used to detect the surface morphology and particle size of three kinds of dental dusts, and the rat dust-stained animal model was established; Giemsa staining and enzyme-linked immunosorbent assay were used to detect rat bronchoalveolar lavage The total number of white blood cells, the number of macrophages and the content of interleukin-6 and interleukin-16 in the fluid; HE staining was used to observe the pathological changes of lung tissue sections.

　　Results The surface morphology and particle size distribution of the three kinds of dental grinding dust particles were different, and the dust particle size was significantly smaller after fine grinding; compared with the control group, the number of white blood cells, the number of macrophages, and interleukin-6 in each dust group The content of interleukin-16 was significantly increased, the inflammatory cells in the lung tissue of rats were infiltrated significantly, and the lung tissue structure was destroyed and fibrosis; Compared with the non-fine grinding group, the inflammatory cells of the Vitalin and zirconium dioxide dust fine grinding group were Inflammatory factors increased significantly.

　　Conclusion The three types of dental dust before and after grinding can cause inflammatory damage and early fibrosis in the lung tissue of rats exposed to pneumoconiosis. Vitalin and zirconium dioxide dust after fine grinding are more inflammatory to the lung tissue of rats than before grinding. Damage is enhanced.