动物造模,巨细胞病毒 z-bio 2022-01-04 237

　　Objective To study the inhibitory effect of cytomegalovirus (CMV) on the immune response of mice implanted with polymethyl methacrylate (PMMA).

　　Method: Inoculate the virus suspension into the abdominal cavity to construct a mouse model of CMV infection. 7 days after virus inoculation, PMMA was implanted into the back of the mouse subcutaneously; flow cytometry was used to detect the percentage of monocytes/macrophages, CD4+ T cells, CD8+ T cells and regulatory T cells (Treg cells) in the peripheral blood Proportion; Enzyme-linked immunosorbent assay (ELISA) method to detect tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) content.

　　Results Compared with the implanted control group, the ratio of MI monocytes/macrophages in the peripheral blood of the implanted CMV group decreased by 73.18% (=5.896, P=0.004), and the ratio of M2 monocytes/macrophages It increased by 2.18 times (t=7.971, P=0.001), the ratio of CD4/CD8 T cells decreased by 42.91% (t=6.468, P=0.003), and the ratio of Treg cells increased by 2.49 times (t=4.495, P =0.011). Compared with the implanted control group, the concentration of TNF-α and IL-1β in the peripheral blood of the implanted CMV group decreased by 71.65% and 57.95%, respectively (t=7.236, P=0.019, t=7.543, P=0.002)

　　Conclusion CMV can significantly inhibit the immune response after PPMA implantation, which provides a theoretical basis for antiviral treatment after implantation of oral biomaterials in CMV-infected patients.