动物造模 z-bio 2022-01-04 551

　　Objective To study the improvement of acupuncture on the symptoms of insomnia rats and its influence on spontaneous activities, and to explore its possible mechanism.

　　Methods The insomnia rat model was established by intraperitoneal injection of p-chlorophenylalanine (PCPA), and they were randomly divided into insomnia group, acupuncture group and western medicine group, each with 10 rats, and a control group with 10 rats. The acupuncture group and the western medicine group were treated with acupuncture and diazepam tablets respectively for intervention. The insomnia group and the control group were treated with normal saline after fixation for 7 days. Open field test to detect spontaneous activity; pentobarbital sodium synergistic sleep test to detect sleep latency and sleep time; RT-qPCR to detect the levels of suprachiasmatic nucleus clock genes clock and period2 (per2); HPLC to detect hypothalamic γ-aminobutine Acid (GABA) and glutamic acid (Glu) content; Western blot detection of hypothalamic GAD and GS protein expression levels.

　　Results The rats in the model group showed typical symptoms of insomnia. Compared with the model group, the acupuncture group and the western medicine group increased the horizontal and vertical movement scores, shortened the sleep latency, prolonged sleep time, increased the relative expression of clock and per2 mRNA in the suprachiasmatic nucleus, decreased the level of Glu in the hypothalamus, and decreased the level of GABA. Increased, Glu/GABA decreased, the relative expression of GAD protein increased, and the relative expression of GS protein decreased (P<0.001, P<0.01, P<0.05); the="" above-mentioned="" indicators="" of="" acupuncture="" group="" and="" western="" medicine="" in="" difference="" was="" not="" statistically="" significant="" p="">0.05).

　　Conclusion Acupuncture can improve the symptoms and spontaneous activities of insomnia rats to a certain extent, and may play an effect by regulating GABA-Gln.