动物造模,免疫抑制 z-bio 2022-01-04 276

　　Objective To investigate the effect of α-mangostin on the immune function of cyclophosphamide-induced immunosuppressive mice, and to provide experimental evidence for subsequent mechanism research and the development of new drugs that increase immune function.

　　Method The mice were randomly divided into 5 groups, each with 10 animals: blank control group, immunosuppressive group, α-mangostin high-dose group, middle-dose group and low-dose group. In the first 7 days, except for the blank control group, the other 4 groups were given cyclophosphamide (CTX) to create an immunodeficiency model; after 14 days, the blank control group continued to be given normal saline via the stomach, and the immunosuppression group was given intragastric administration. Stomach corn oil, the three dose groups were given different doses of α-mangostin. The mice were sacrificed 24 hours after the last administration, and the delayed-type allergy (DTH) test, serum hemolysin test (HC50), macrophage phagocytic function test (carbon clearance test) and immune organ thymus and spleen index test were performed. Killer cell (NK cell) activity measurement (MTT method), measurement of peripheral blood white blood cell count, and splenic lymphocyte proliferation experiment, to study the effect of α-mangostin on the immune function of mice.

　　Result The mouse immunosuppression model was successfully created. After intragastric administration of α-mangostin, the three dose groups can increase the thymus and spleen index, half hemolysis value, splenic lymphocyte proliferation rate, and NK cell activity of immunosuppressed mice to varying degrees. Among them, the high dose group has total α-mangostin. The gavage dose of Sulfone is 100 mg / (kg In delayed-type allergy, except for the high-dose group and the blank control group and immunosuppressive group, there are significant differences (P<0. p="">0.05); in the peripheral blood white blood cell count determination experiment, the high-dose group and the immunosuppression group The immunosuppression group has a very significant difference (P<0. p="">0.05); in the carbon clearance experiment, each dose group has no statistical significance compared with the blank control group and the immunosuppression group (P> 0. 05).

　　Conclusion Mangostin has a regulatory effect on the immune function of immunosuppressed mice, and its optimal dose for improving the immune function of immunosuppressed mice is more biased towards the high-dose group, which is dose-dependent within a certain range.