动物造模,子痫前期大鼠肾损伤 z-bio 2022-01-04 667

　　Objective To investigate the effect of resveratrol (RSV) on the lymphotoxin analogue (LIGHT)/herpes virus interventional body (HVEM) pathway in preeclampsia (PE) rats and its protective effect on renal injury.

　　Methods 50 SD pregnant rats were randomly divided into normal pregnant rats (Normal group), preeclampsia model group (PE group), low resveratrol (RSV) (50 mg/kg), high (200 mg/kg) ) Dose group, LIGHT/HVEM pathway blocker (lymphotoxin β receptor, LTβR-Ig fusion protein, 1600 μg/kg, LTβR-Ig group), 10 rats in each group. Except for the Normal group, rats in the other groups were intraperitoneally injected with nitroso-L-arginine methyl ester (L-NAME, 300 mg/kg, 5 days in total, once a day) to establish a rat model of preeclampsia, and the model was successfully established After the administration was started, each dose group of RSV was given the corresponding dose of RSV solution by gavage, the LTβR-Ig group was injected with the LTβR-Ig solution via the tail vein, the Normal group and the PE group were given the same amount of normal saline by gavage + tail vein injection, and each group was consecutive The drug was administered for 5 days, once a day. After the last administration, the rat's urine was collected for 24 hours, and the blood was taken, and the enzyme-linked immunosorbent (ELISA) kit was used to detect the renal function indicators of serum creatinine (Scr), blood urea nitrogen (BUN) and 24-hour urine protein in the urine. ; Hematoxylin-Eosin (HE) staining was used to detect the pathological changes in the kidney tissues of rats in each group; real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) was used to detect the relative levels of LIGHT and HVEM mRNA in kidney tissues; Western blotting (Western Blot) to detect the relative expression levels of LIGHT, HVEM, nuclear transcription factor-κB (NF-κB), and interleukin-6 (IL-6) in kidney tissue.

　　Results Compared with the Normal group, rats in the PE group developed pathological damage such as diffuse proliferation of glomerular endothelial cells, serum Scr, BUN, and 24-hour urine protein in urine, renal tissue LIGHT mRNA and protein, HVEM mRNA and protein, The expression of NF-κB and IL-6 protein increased (P<0.05); compared with the PE group, the renal tissue pathological damage of rats in the RSV low-dose, high-dose group, and LTβR-Ig group was relieved, and serum Scr, BUN and urine The 24-hour urine protein content, renal tissue LIGHT mRNA and protein, HVEM mRNA and protein, NF-κB, IL-6 protein decreased in 24 hours (P<0. rsv="" r-ig="" p="">0 . 05).

　　Conclusion RSV can inhibit the expression of LIGHT/HVEM pathway proteins in the kidney tissue of PE rats, and improve PE kidney injury.