动物造模 z-bio 2022-01-04 422

　　Objective To explore the effect and mechanism of Shenqu Xiaoshi oral liquid on gastrointestinal motility in mice with functional dyspepsia (FD).

　　Method 40 KM mice were randomly divided into normal group, Shenqu Xiaoshi oral liquid low, medium and high dose groups, and the blood routine and liver and kidney function indexes of the mice were tested. Another 50 KM mice were randomly selected as the normal group, and the remaining mice were used irregular eating + L-arginine (L-Arg) method to construct FD animal models, and then randomly divided into model group, Shenqu Xiaoshi oral Liquid low, medium and high-dose groups; record the weight of the mice, calculate the residual rate and intestinal advancement rate of the mouse, observe the pathological changes of the stomach tissue, use real-time fluorescent quantitative PCR (qRT-PCR) and Western blot (Western blot) ) To detect the expression of endoplasmic reticulum stress factor inositol-requiring enzyme 1 (IRE1) and tumor necrosis factor receptor related factor 2 (TRAF2) in mouse gastric tissue.

　　Results Each group of mouse white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), platelets (PLT), lymphocytes (LYM), monocytes (MONO), neutrophils (NEU), aspartic acid Comparison of aminotransferase (AST), alanine aminotransferase (ALT), total bile acid (TBA), urea nitrogen (BUN) and creatinine (CRE), there was no statistically significant difference between the groups (P>0.05) The mucosal layer, submucosa, muscle layer and serosal layer of the gastric tissue of each group of mice have clear structures, and no obvious pathological changes were seen; compared with the model group, the gastric discharge of Shenqu Xiaoshi oral liquid low, medium and high dose groups Emptying rate and small intestine advancement rate were significantly increased, and the expression of mRNA and protein of IRE1 and TRAF2 in gastric tissue was significantly decreased (P<0.05).

　　Conclusion Shenqu Xiaoshi Oral Liquid has no significant effect on blood routine and liver and kidney function of normal mice. It can significantly improve the gastrointestinal motility function of FD mice, which may be related to the down-regulation of the expression of endoplasmic reticulum factors IREl and TRAF2.