动物造模 z-bio 2022-01-05 552

　　Objective To investigate the regulatory effect of Huangqi injection on immune cells and inflammatory factors in db/db mice and its possible mechanism.

　　Method C57BL/6 mice were used as a control group, and dbdb mice were randomly divided into a diabetes group and a diabetic Huangqi injection treatment group. The mice in the control group and the diabetic group were intraperitoneally injected with 100 μL of PBS each day, and the mice in the diabetic astragalus injection treatment group were injected with 100 μL of astragalus injection daily for 12 weeks. After 12 weeks, the number and classification of peripheral blood leukocytes were detected; the expression of interleukin-10 (IL-10) and tumor necrosis factor alpha (TNF-α) in serum was detected by ELISA; the expression of interleukin-1β (IL) in mouse kidney was detected by real-time PCR. -1β), TNF-α and human monocyte chemoattractant protein-1 (MCP-1) mRNA expression; flow cytometric detection of the percentage of regulatory T cells (Tregs) in the spleen.

　　Results The number of white blood cells, the percentage of neutrophils and the percentage of monocytes in the peripheral blood were significantly higher in the diabetes group than in the control group, and the astragalus injection treatment group was significantly lower than that in the diabetes group. The serum Ⅱ-10 in the diabetes group was significantly lower than that in the control group, and the TNF-α was significantly higher than that in the control group. The IL-10 of the Huangqi injection treatment group was significantly higher than that of the diabetes group, while the TNF-α was significantly lower than that of the diabetes group. The mRNA expression of IL-1β, MCP-1 and TNF-α in the diabetes group was significantly higher than that in the control group, and the Astragalus injection treatment group was significantly lower than the diabetes group; the number of Tegs cells in the diabetes group was higher than that of the control group, while the Astragalus injection treatment group It was significantly higher than the control group.

　　Conclusion Astragalus injection can reduce the number of inflammatory cells in db/db mice, increase the number of Tegs cells, and reduce the expression of pro-inflammatory factors IL-1β, MCP-1 and TNF-α; delay the occurrence of diabetic nephropathy (DN).