动物造模 z-bio 2022-01-06 588

　　Objective Cyclophosphamide was administered intravenously to pregnant rabbits to observe its toxic effect and performance on pregnant rabbit embryos and fetuses, and to provide reference for the selection of cyclophosphamide as a positive control.

　　Method The pregnant rabbits were randomly divided into a control group [0.9% sodium chloride injection, GD11 (GD, pregnancy day), i.v] and a cyclophosphamide group (cyclophosphamide 30 mg/kg body weight, GD11, i.v). GD28 euthanized the pregnant rabbits, took the fetuses by cesarean section, and checked the appearance, internal organs and bones of the fetuses.

　　Results The cyclophosphamide group had no significant effect on the rabbit's reproductive function and embryo formation, and could cause distortion of appearance, internal organs and bones. The rate of fetal appearance cleft palate deformity was 32.95%; the rate of visceral variation was 10.23%; the rate of skeletal deformity was 23.37%.

　　Conclusion In New Zealand, intravenous administration of cyclophosphamide 30 mg·kg-1 without GD11 can cause fetal malformations. The type and proportion of malformations are appropriate and can be used as a positive control for reproductive tests.