动物造模 z-bio 2022-01-06 371

　　Objective To investigate the differences in the effects of non-professional processing of wheat straw bedding, corncob bedding, shavings bedding and paper bedding on the learning and memory abilities of SPF Kunming mice (KM), as well as the effects of different bedding on the behavior of mice Influence.

　　Methods Twenty-four SPF-grade KM mice were selected and fed with malt extract, wood shavings, corn cobs and paper bedding for 8 weeks. The MORRIS water maze behavior experiment was carried out to investigate the changes in behavior and the ability of learning and memory. difference.

　　Results The increase in learning and memory ability in the wheat straw group was significantly higher than that in the shavings group (P<0.05); during the water maze experiment, there was no significant difference in weight gain between the groups. The results of the positioning navigation experiment showed that the escape latency of the wheat straw group on the 5th day was significantly lower than that of the other three groups (P<0.05), and there was no significant difference in the rest of the time period; the results of the space exploration experiment showed that the number of times the mice in the wheat straw group passed through the original platform position, The swimming time was longer than the other three groups.

　　Conclusion Different litter has little effect on the body weight of KM mice in the water maze experiment. The effect of wheat straw litter on the learning and memory ability of KM mice is better than that of shavings litter and corncob litter. It is recommended to use wheat straw litter as experimental animals Promote the use of contact bedding.