动物造模,肠道菌群多样性 z-bio 2022-01-06 494

　　Objective To investigate the influence of N-acyl homoserine lactones (AHLs), which is a signal molecule for the flora sensing, on the composition of the intestinal microflora of mice, and to provide a theoretical basis for the disturbance of the intestinal microecology under infection.

　　Methods After intraperitoneal injection of 3-oxo-C10-HSL molecules into mice, samples were taken in the duodenum and jejunum of mice by swab smearing method at 24 hours, and the samples were sampled for 16S using the Illumina Hiseq 2500 high-throughput sequencing platform. rDNA sequencing, and analysis of the classification and diversity of microbial flora.

　　Results A total of 22 bacterial phyla and 109 bacterial genera were detected in the intestines of experimental mice; the bacterial flora in different parts of the intestines of the control group and the 3-oxo-C10-HSL treatment group were classified as Firmicutes (>50%). ) And Proteobacteria (>20%), but there are significant differences in the composition of the two groups at the level of the genus. The dominant bacteria in the control group are Staphylococcus, Escherichia-Shigella, Lactococcus, Pseudomonas The dominant bacteria in the 3-oxo-C10-HSL treatment group are Enterococcus, Escherichia-Shigella, and Staphylococcus: analysis of diversity and abundance suggests that the intestines of the 3-oxo-C10-HSL treatment group The diversity of the tract flora has increased, the abundance of major dominant bacteria such as Escherichia coli has increased significantly, and the colony abundance in the jejunum is the highest.

　　Conclusion AHLs has little effect on the composition of the intestinal flora of mice, but has a significant effect on the changes in the structure of certain specific groups within the flora.