动物造模,发性癫癎模 z-bo 2022-02-19 545

　　(1) Reproduction method. Determine the degree of onset of mice before the experiment, and select qualified animals for the experiment. Place the animal on a small wooden board, gently throw it 10-15cm into the air, and repeat it several to several 10 times to cause the attack. This difference is due to the different attack threshold of each mouse. It is generally stipulated that the author is tossed repeatedly 80 times without sending the author, which is considered to be an insensitive mouse and not used in the experiment.

　　(2) Model characteristics The seizures of epileptic mice can be divided into two stages: the outbreak period and the late outbreak period. In the process of throwing into the air, the mouse suddenly made a chirping sound. At this time, immediately stop throwing and observe carefully. The mouse quickly entered a state of temporary immobility, the tail was bent upwards, and then ran and entered the state of explosive epilepsy; then into the late stage of the outbreak, the mouse maintained the kangaroo posture, the tail was raised, the forelimbs were raised, and the upper and lower positions Contracture movements, chewing activities and head turning to the side, etc., accompanied by balance disorders, salivation, and incontinence. The entire seizure process lasts 13-20s, and then enters the convulsive refractory period of about 30min. At this time, giving any stimulation does not cause the seizure.

　　(3) Comparative Medicine Epilepsy-like mice are pure-bred mice obtained from DDY mice through 12 generations of inbreeding and breeding. It is an internationally recognized model of hereditary epilepsy. Such mice cause seizures when the front court is stimulated. For example, repeatedly throwing it into the air, shaking and turning repeatedly, etc., can cause seizures. Animals start to attack at 5-8 weeks after birth, reach a peak when they are sexually mature, and then remain for life. The epileptic mouse seizures start in the hippocampus and deep temporal lobe, and then spread to other parts of the brain. The seizures are similar to human temporal lobe epilepsy in many ways. Therefore, it is a temporal lobe epilepsy. Model of epilepsy.