创伤性脑损伤模型,动物造模 z-bio 2022-01-18 439

　　Objective: To investigate the changes in the content or activity of nuclear factor E2 transcription-related factor 2 (Nrf2) and anti-oxidative stress-related factors in a rat model of traumatic brain injury, and to study the protective effect of curcumin on rat brain injury and anti-oxidative stress mechanism.

　　Methods: Twenty SPF male SD rats were selected and divided into normal control group, brain injury model group (TBI group), brain injury solvent group (TBI+S group), brain injury curcumin-treated group (TBI+C group) , 5 in each group. The normal control group was only given anesthesia and normal saline treatment, and the TBI group, TBI+S group and TBI+C group were all used the free-fall traumatic brain injury modeling device for modeling, and then were given the same amount of Physiological saline, DMSO solvent (0.05%) and curcumin (5 mg/kg) were treated. All the rats were sacrificed 1 day later, and the brain tissue was collected to extract RNA and protein. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect Nrf2 The mRNA expression of rat brain tissue was detected by Western Blot, and the expression of Nrf2 protein was detected by Western Blot. The content of malondialdehyde (MDA) and reduced glutathione (GSH), catalase (CAT) in rat brain tissue homogenate were detected by chemical colorimetry The activities of superoxide dismutase (SOD) and superoxide dismutase (SOD), and the contents of inducible nitric oxide synthase (iNOS) and heme oxygenase-1 (HO-1) were detected by enzyme-linked immunosorbent assay (ELISA).

　　Results: Compared with the normal control group, the mRNA and protein expressions of Nrf2 in the brain tissue of TBI group and TBI+S group were significantly increased, the content of MDA, the activities of iNOS and HO-1, the content of GSH, the activities of SOD and CAT were increased. Compared with the TBI group and the TBI+S group, the mRNA and protein expression levels of Nrf2 in the TBI+C group were significantly decreased, and the MDA content, iNOS and HO-1 activity were all decreased , GSH content, SOD and CAT activities all increased, and the difference was significant (P<0.05), while there was no significant difference between the TBI group and the TBI+S group (P<0.05).

　　Conclusion: Curcumin has an anti-oxidative stress effect on brain injury rats. It can reduce the expression of Nrf2 and change the body's anti-oxidative damage-related indicators, which may play a protective effect on TBI brain tissue.