动物造模 z-bo 2022-02-02 346

　　The lateral hydraulic impact brain damage model quickly injects a certain amount of saline into the cranial cavity to cause the deformation and displacement of the brain tissue to cause TBI. The hydraulic device is composed of a circular liquid column (64cm in length and 4.5cm in diameter), strike frame, oscilloscope and pressure sensor. One end of the circular liquid column is connected with a piston, and the other end is connected with a blow tube and a pressure sensor. At the time of injury, the entire pipeline system was filled with 37°C normal saline and then closed. The pressure generated by the hammer hitting the piston was transmitted to the cranial cavity through the liquid in the hitting tube and acted on the tissues. Specific steps are as follows:

　　(1) The rats were fed alone 3 days in advance and fasted for 8 hours before surgery. During the experiment, 3.6% chloral hydrate was used to inject anesthesia into the abdominal cavity at a dose of 1 ml per 100 g, and fixed in the prone position on the bracket of the stereotaxic instrument.

　　(2) Make a median cut of the first skin, 3mm behind the left and right coronal suture and 2mm next to the sagittal suture respectively, and drill the skull with a dental drill. The diameter of the bone window is about 3ml. Fix the sterile blow tube with self-setting dental tray powder in the bone window.

　　(3) A bone window was opened 3mm behind the contralateral coronal suture and 2mm next to the sagittal suture, with a diameter of about 15mm, embedded in a silicone tube 15mm, and monitored intracranial pressure, while monitoring the blood pressure, heart rate, and respiration of the rat.

　　(4) Establish a rat model of graded craniocerebral injury according to the impact force. Different blows are given according to the grouping situation (the size of the blow is determined according to the pre-experiment): 1313～3313kPa or less for the light injury group; 3314～5017kPa for the stale injury group; 5018～7313kPa for the severe injury group; 7314kPa for the severe injury group ; The control group was not given impact, but the operation procedure was exactly the same.

　　The model's injury force is quantitative and accurate, and it can directly reflect the pressure on the brain tissue. Good reproducibility, can replicate mild, moderate and severe head injuries. Currently, this model is widely used in neuropathology, neurobiochemistry, neuroelectrophysiology, neurotransmitters and receptors, neurobehavioral functions, brain energy metabolism, cerebral blood flow, and drug efficacy judgments after head injury. However, the injury mechanism of this model is not completely consistent with the human injury mechanism, and the instrument is complex and expensive. Basically, it is self-developed and assembled in China.