动物造模 z-bio 2022-01-18 335

　　Objective: By transplanting the feces of pigs with different feed utilization efficiency into pseudo-sterile mice, to explore the law of its influence on the growth performance of the recipient mice, and to reveal the mechanism preliminarily.

　　Methods: 36 Du×Chang×Large barrows of about 28kg were used for 42d single-cage feeding (ad libitum) and their feed conversion efficiency was measured. At the end of the experiment, the 36 pigs were divided into high, medium, and medium according to the feed conversion efficiency. In the low 3 group, fresh feces of the three groups of pigs were collected and transplanted into mice treated with broad-spectrum antibiotics by gavage to monitor the changes in the growth performance of the mice after fecal transplantation. The total fecal collection method was used to determine the high feed utilization efficiency and high feed utilization efficiency. The nutrient digestibility of pigs in the middle and low groups was analyzed, and the fecal microbial composition of pigs and mice was analyzed and compared by 16S rRNA V3-4 variable region amplification sequencing.

　　RESULTS: After transplanting feces from pigs with different FUEs into pseudo-sterile mice treated with broad-spectrum antibiotics, the mice reproduced the growth performance phenotype of pigs; in terms of gut microbial composition, pigs with high FCEs and the recipient mice with high growth performance, the species richness and microbial diversity of gut microbes were relatively high; the total energy digestibility (P=0.01) of pigs with high feed utilization efficiency increased significantly, and the abundance of Brevibacterium aerogenes was significantly higher. In addition, the abundance of Enterococcus and Akkermansia in recipient mice after fecal transplantation was also significantly higher than that in recipient mice with low feed utilization efficiency, indicating that gut microbes play an important role in efficient energy utilization. effect.

　　CONCLUSION: Transplanting feces from pigs with different feed utilization efficiencies can directionally alter the species richness, microbial diversity and growth performance of gut microbes in pseudo-sterile mice. The abundance of microbes associated with efficient energy utilization is relatively high in the gut.