How to prepare an animal model of chronic exercise fatigue?

  (1) Method of replication: SD male rats with a weight of 200-220g were selected. Using 7 weeks of high-intensity treadmill exercise, starting from the first week of training, the weekly speed is increased at 15m/min, 22m/min, 27m/min, 31m/min, 35m/min. Train for 20 minutes a day, 5 days a week, for a total of 5 weeks. After the 5th week, two exercise intensity models were established: in the 6th and 7th weeks, the general training group was run at a speed of 35m/min on a treadmill with a slope of 0 for 20 minutes; the intensive training group was in the 6th and 7th week. Weekly, run for 25 minutes every day under the same conditions to establish a long-term incremental load exercise fatigue animal model. At the end of the experiment, hemoglobin, blood lactic acid, blood urea, urine protein and other indicators were detected.

  (2) Model characteristics The chronic exercise fatigue animal model is a group of symptoms that causes the animal to stay in high-intensity exercise for a long time and the fatigue accumulation and functional state decline. It has a certain reference for the study of human body fatigue caused by long-term excessive exercise. value. When selecting testing indicators, we should not only pay attention to the relative independence of indicators, but also consider the best combination of multiple indicators.