动物造模,心梗 z-bio 2022-01-19 408

　　Objective: To study the effect of intraosseous local single injection of low-dose simvastatin on angiogenesis and cardiac function in rats after myocardial infarction.

　　Methods: Wistar rats were randomly divided into sham operation group, myocardial infarction model group and intraosseous simvastatin group (n=12). A rat model of myocardial infarction was established by ligation of the left anterior descending coronary artery. After 24 hours, the experimental group was given a single injection of simvastatin 0.5 mg into the left tibia. After 4 weeks, the left ventricular function was evaluated by small animal echocardiography, and the myocardial infarction area was calculated by triphenyltetrazolium chloride (TTC) staining. Fluorescence staining was used to detect local angiogenesis.

　　RESULTS: Echocardiography showed that the left ventricular systolic function decreased significantly 4 weeks after myocardial infarction, and intraosseous injection of simvastatin did not significantly improve left ventricular function in rats after myocardial infarction; TTC staining showed that the myocardial No significant reduction in infarct size was found; immunofluorescence staining showed no significant increase in myocardial vascular density in the intraosseous simvastatin group.

　　Conclusion: A single intraosseous injection of low-dose simvastatin (0.5 mg) 24 hours after myocardial infarction in rats does not significantly improve myocardial infarction size, angiogenesis and cardiac function.