动物造模 z-bio 2022-01-21 692

　　Objective: To investigate the effect of miR-424 on the migration and invasion of non-small cell lung cancer cell line A549.

　　Methods: RT-PCR was used to detect the expression of miR-424 in lung cancer cells NCI-H460, NCI-H1975, NCI-H446, A549, NCI-H1299, NCI-H157 and human embryonic lung fibroblasts MRC-5, liposome LipofectamineTM2000 miR-424 inhibitor and miR-424 NC were transferred into A549 cells. After 48 h, the expression of miR-424 was detected by RT-PCR method, cell viability was detected by CCK-8 method, cell migration ability was detected by scratch assay, and cells were detected by Transwell assay. Invasion ability, the expression of matrix metalloproteinase 2 (MMP2), MMP9, transforming growth factor-β1 (TGF-β1) and p-Smad3 was detected by western blot.

　　Results: The expression of miR-424 in lung cancer cells NCI-H460, NCI-H1975, NCI-H446, A549, NCI-H1299, NCI-H157 [(1.78±0.13), (1.69±0.10), (1.89±0.18), (2.88±0.27), (2.52±0.20), (2.49±0.23)] expression was significantly higher than that of miR-424 in human embryonic lung fibroblast MRC-5 (0.58±0.05) (P<0.01) . Compared with the miR-424 NC group, the miR-424 inhibitor group had significantly lower miR-424 expression (P<0.01), cell viability (P<0.01), cell migration and invasion abilities (P<0.01), MMP2, MMP9 , TGF-β1 and p-Smad3 expressions were significantly down-regulated (P<0.01).

　　Conclusion: The down-regulated expression of miR-424 can inhibit the migration and invasion of A549 cells by inhibiting the TGF-β1/Smad3 signaling pathway.