动物造模,基因敲除 z-bio 2023-01-29 419

　　OBJECTIVE: To establish a related mouse knee osteoarthritis model by knocking out SIRT1 gene, and to observe the differences in the morphology of cartilage tissue sections with different single staining or compound staining techniques.

　　Methods: The knee joint tissue samples of mice were divided into 2 groups: SIRT1-/- mouse sham operation group (n=6, group A), SIRT1-/- mouse osteoarthritis model group (n=6, B group) ). Knee osteoarthritis model was established by anterior cruciate ligament transection and medial meniscectomy, HE staining, Safranin O-fast green staining, Safranin O-Alcian blue staining, Safranin O staining, Fast green staining, Alli The morphological changes of knee articular cartilage were observed by neo-blue staining and 6 single staining or compound staining methods.

　　Results: Safranin O-fast green staining and safranin O-alcian blue staining were used to observe the cell morphology of chondrocytes, cartilage layered structure, display of type II collagen fibers, tide line and changes in subchondral bone. The effect is better; while Safranin O staining and Alcian blue staining have certain advantages in observing cartilage tissue loss.

　　Conclusion: In the observation of cartilage tissue sections of knee osteoarthritis of SIRT1 knockout mice, compared with single staining, compound staining has more obvious advantages in obtaining various information of cartilage structure.