动物造模,脑缺血再灌注损伤 z-bio 2023-01-18 708

　　Objective: To investigate the effect of hydrogen-rich solution on the apoptosis of hippocampal neurons and the expression of PI3K/Akt/FoxO1 signaling pathway induced by cerebral ischemia-reperfusion in rats.

　　Methods: The middle cerebral artery of rats was occluded by suture method, and a rat model of focal cerebral ischemia/reperfusion (I/R) was established. SD rats were randomly divided into sham operation group (Sham group), ischemia/reperfusion group Perfusion group (I/R group) and hydrogen-rich solution treatment group (HRS group), 10 animals in each group; 24 h after reperfusion in each group, serum inflammatory factors IL-6, TNF-α and IL-1β were detected by ELISA The changes of hippocampus were observed by HE staining, the apoptosis of brain tissue was observed by TUNEL method, and the protein expressions of p-PI3K, Akt, caspase-3 and FoxO1 in hippocampus were detected by Western blot method.

　　Results: Compared with the Sham group, the pyramidal cells in the I/R group were loosely arranged, a large number of pyramidal cells died, the hippocampal apoptotic cells increased, and the expressions of serum inflammatory factors IL-1β, IL-6, TNF-α, and TNF-α were significantly increased (P < 0.05), the expressions of p-PI3K, Akt, and caspase-3 in brain tissue were significantly increased, and FoxO1 protein was decreased, and there was a statistical difference between the two groups (P < 0.05); compared with the I/R group, the HRS group Inflammatory factors were significantly decreased; the expression levels of p-PI3K, Akt, and caspase-3 were significantly decreased, and FoxO1 protein was increased (P<0.05).

　　Conclusion: Hydrogen-rich solution can alleviate brain injury caused by ischemia-reperfusion, and the mechanism may be related to PI3K/Akt/FoxO1 signaling pathway.