动物造模 z-bio 2022-01-24 757

　　Objective: To investigate the localization and expression of vitamin D receptors in the duodenum of C57BL/6 mice, and to study the correlation between vitamin D system and intestinal development.

　　Methods: Using real-time quantitative PCR (quantitative PCR, qPCR), hematoxylin and eosin staining, immunofluorescence staining, Western blotting (Western blotting, WB) detection techniques, the expression of VDR in the duodenum of C57BL/6 mice was detected. Do qualitative and quantitative analysis.

　　RESULTS: The expression of VDR mRNA in the duodenal tissue of C57BL/6 mice reached a peak on the 21st day of birth. In addition, VDR was mainly distributed in the cytoplasm of duodenal epithelial cells and smooth muscle cells of C57BL/6 mice. WB detection of tissue samples showed that there was no obvious nuclear translocation of VDR during duodenal development in C57BL/6 mice.

　　Conclusion: From the perspective of molecular biology, the expression rules of VDR in the duodenum of C57BL/6 mice at different growth and development stages were analyzed, which laid a theoretical foundation for understanding and improving the role of VDR in the intestine.