动物造模,实验性自身免疫性脑脊髓炎 z-bio 2023-01-30 577

　　Objective: To observe the effect of different concentrations of baicalin (baicalin) on experimental autoimmune encephalomyelitis mouse model, and to study its preliminary mechanism.

　　Methods: A mouse model of experimental autoimmune myelitis was established. On the 3rd day after immunization, high and low doses of baicalin were given by gavage, once a day, for a total of 20 days. The activity of mice was scored for neurological function, TUNEL staining was used to detect the apoptosis of spinal cord tissue, ATP level detection kit was used to detect the level of ATP content in spinal cord tissue, and Western blot was used to detect Bax, Bcl-2, cleaved cas-9 and cleaved cas-3 protein expression levels.

　　Results: Baicalin can improve the neurological function of mice with experimental autoimmune encephalomyelitis and delay the onset time; after the intervention of baicalin, Tunel staining showed that the number of apoptotic cells in spinal cord tissue decreased, and the level of ATP decreased. Western blotting results It showed that the protein expression of Bcl-2 increased significantly, and the protein expression of Bax, cleaved cas-9 and cleaved cas-3 decreased significantly.

　　Conclusion: Bachinoside can inhibit apoptosis by inhibiting mitochondrial endogenous apoptosis pathway, protect mitochondria, and improve neural function in mice with experimental autoimmune encephalomyelitis, which provides an experimental theoretical basis for disease prevention.