动物造模,高血压 z-bo 2022-02-02 402

　　[Operation steps] Commonly used animals are adult SD or Wistar rats, 120-150g rats, anesthetized with diazepam 5mg/kg and ketamine 50mg/kg, fixed in prone position, put a cotton gauze about 2~3cm under the abdomen, abdominal surgery Prepare the skin and disinfect the skin. Cut the skin along the midline of the spine from the 10th thoracic vertebra to the 3rd lumbar vertebra. Use small vascular forceps to separate the muscles from the lower part of the abdomen 1.5-2cm below the left side rib and 1cm from the spine. Squeeze the kidney from its own mouth, carefully peel off the kidney from the surrounding tissues, cut the self-made double-layer latex film into an "X" shape, wrap the kidney crosswise around the kidney door, then cut it on the opposite side, take out the right kidney, and separate After resection, and finally suture the surgical incision in layers. Subcutaneous injection of 10,000 to 20,000 units of penicillin G. After the operation, 1% sodium chloride solution can be added as a promoting factor. After about 20 days, more than 70% of the rats have persistent high Blood pressure and systolic blood pressure can generally increase by more than 50%.

　　Bandaging of foreign bodies outside the kidney can cause peri-nephritis, forming a layer of cellulose sheath outside the kidney, compressing the renal parenchyma, causing renal tissue ischemia, increasing the formation of renin, and then increasing the blood angiotensin content and blood pressure. high. In addition to the self-made double-layer latex film and cellophane, the kidney wrappers can also use silk cloth, latex, collodion and other materials. The external tying of the kidney should be tight enough, but not to break the tissue. The above is a 1 kidney 1 tie type operation. If you want to make 2 kidneys and 1 tie, you do not need to remove the other kidney after bandaging one kidney, and 10% to 20% of the animals develop hypertension 3 to 6 months after surgery. If 2 kidneys and 2 ties are made, one kidney will be bandaged and the other kidney will be bandaged in the same way. Over 70% of the animals will develop hypertension 3 to 4 weeks after surgery.

　　[Result analysis] Renal hypertension (especially renovascular hypertension) model is a commonly used hypertension model. It has the following characteristics: pathophysiology and human hypertension have many similarities; surgical operation is relatively simple; high blood pressure is stable, and the response to antihypertensive drugs is consistent with that of hypertensive patients, and is suitable for antihypertensive drug screening and efficacy evaluation.