动物造模,高血压 z-bo 2022-02-02 425

　　[Operation steps] 100～150g male SD rats were anesthetized by intraperitoneal injection of Diazepam 5mg/kg and ketamine 50mg/kg, and left nephrectomy was performed through a median abdominal incision. After the operation, rats were injected subcutaneously with deoxycorticosterone acetate (D()CA) 50mg/kg, once a day for 5 days a week for 5 weeks, while drinking 1% NaCl solution, and drinking water after stopping the administration . About 50% of the rats' blood pressure increased after 1 week of administration, and 70% of rats developed persistent hypertension after 5 weeks of discontinuation of the administration, and those with systolic blood pressure >160mmHg (21.3kPa) were used as experiments.

　　[Precautions] The subcutaneous injection site is on the back and should be replaced frequently. The injection needle should be wiped and disinfected with ethanol to avoid local infection. It is also possible to embed DOCA tablets subcutaneously to create a model. DOCA alone is not easy to cause stable hypertension in rats, and one kidney should be removed in advance and 1% NaCl should be added to cause persistent hypertension. When preparing some hypertension models, NaCl is often used as a promoting factor for the formation of hypertension. In fact, a long-term high-salt diet alone can form chronic hypertension.

　　[Result analysis] Deoxycorticosterone acetate (DOCA) is a mineralocorticoid, which has obvious water and sodium retention effect, which increases extracellular fluid and blood pressure. Subcutaneous injection of DOCA and additional drinking of 1% NaCl solution after one side of the kidney in the rat can form persistent hypertension. There are many types of endocrine hypertension models. The DOCA salt hypertension model is more commonly used. It is easy to prepare, has relatively stable hypertension, and has a response to antihypertensive drugs that is more consistent with hypertensive patients. It is suitable for antihypertensive drug screening and Efficacy evaluation.