动物造模,脓毒症 z-bio 2022-05-31 472

　　Objective: To observe the effect of Trichinella spiralis and its parasite-derived protein on cecal ligation and puncture (CLP)-induced sepsis in mice.

　　Methods: 80 male BALB/c mice were randomly divided into sham operation group, CLP group, Trichinella muscle larvae (ML) pre-infection group, Trichinella muscle larvae body soluble protein (SMP) treatment group and excretion secretory protein (MES) group. ) treatment group. The ML pre-infection group was orally infected with 300 Trichinella muscle larvae 28 days before operation, and the other groups were injected with PBS or SMP (25 μg/bird) or MES (25 μg/bird) by intraperitoneal injection 30 min after surgery, respectively. The postoperative status and 72-hour survival rate of mice were observed, and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea nitrogen (BUN) and creatinine ( Cr) level and TNF-α, IL-6, IL-1β, IL-10, TGF-β levels, and observe the liver and kidney tissue lesions of mice.

　　Results: Compared with the sham operation group, the 72-hour survival rate of the CLP group was decreased, the levels of ALT, AST, BUN and Cr in serum and the levels of cytokines TNF-α, IL-6, IL-1β, IL-10 and TGF-β were significantly increased (P<0.05). The arrangement of the hepatic cords in the liver is disordered, the hepatocytes are edema, some of the glomeruli in the kidney are shrunken, and the renal tubular cells are edema. Compared with the CLP group, the serum levels of ALT, AST, Cr, TNF-α and IL-1β in the ML pre-infection group decreased, while the levels of IL-10 and TGF-β increased (P<0.05). , AST, Cr, TNF-α and IL-1β levels decreased, and TGF-β levels increased (P<0.05); the 72-hour survival rate of MES treatment group was significantly increased, serum ALT, AST, BUN, Cr, TNF-α The levels of α, IL-6 and IL-1β were significantly decreased, the levels of IL-10 and TGF-β were significantly increased (P<0.05), and the structural damage of liver and kidney was significantly alleviated.

　　Conclusion: Trichinella spiralis and its parasite-derived protein can reduce the release of pro-inflammatory factors and promote the release of immunoregulatory factors in the serum of CLP-induced sepsis mice, among which MES has a more significant effect, and can reduce liver and kidney structure and metabolism. functional impairment.