动物造模 z-bio 2022-02-08 439

　　Objective: To explore the effects of different doses of microbial-derived antioxidants on sleep and its antioxidant properties in mice.

　　Methods: 60 male Kunming mice were randomly divided into 4 groups. The low-dose, medium-dose and high-dose groups were fed with 0.5 g/kg body weight, 1.0 g/kg body weight and 1.5 g/kg body weight of microbial antioxidants, respectively. Normal saline was administered by gavage, and the experimental period was 30 d. After the last gavage, the animals in each group were intraperitoneally injected with sodium pentobarbital, and the sleep status of the mice was observed by righting reflex test, and the antioxidant properties of the serum of the mice were detected after the end.

　　Results: Compared with the low and high dose groups, the microbial-derived antioxidants in the middle dose group could significantly prolong the pentobarbital sodium sleep time (P<0.05). Compared with the control group and the low and high dose groups, the middle dose group significantly increased the activity of GSH-Px (P<0.01) and significantly increased the activity of SOD (P<0.05). Compared with the control group, the contents of MDA and 8-ISO-PGF2α in the middle-dose group were significantly decreased (P<0.05).

　　Conclusion: It is suggested that microbial-derived antioxidants can promote the sleep of mice and improve the antioxidant capacity of the body, and the effect is the most significant at the dose of 1.0g/kg body weight.