动物造模,神经元细胞 z-bio 2022-03-17 400

　　Objective: To establish a method to evaluate the effect of paeoniflorin on the concentration of free Ca2+ in rat dorsal root ganglion neurons.

　　Methods: The rat dorsal root ganglion (DRG) was obtained by microdissection, digested with trypsin, sieved, and incubated and purified with DF-12 and anti-filament medium alternately to obtain primary rat DRG neuron cells. Cellular immunofluorescence technique was used to determine the cell purity of DRG neurons; laser confocal microscopy imaging technique was used to observe the change of intracellular Ca2+ fluorescence intensity, and to analyze the change rate of Ca2+ fluorescence intensity. concentration and its effect on capsaicin receptors.

　　Results: The purity of DRG cells isolated by the above method can be as high as 95%. Capsaicin can inhibit the increase of intracellular Ca2+ by blocking the effect of capsaicin-activated transient receptor potential channel. Paeoniflorin exhibits similar performance to capsaicin. It can block the influx of extracellular Ca2+.

　　Conclusion: Paeoniflorin may inhibit the large increase of Ca2+ in DRG cells by acting on TRPV1 channel. This method can be used to evaluate the effect of drugs on the concentration of Ca2+ in DRG cells of rats.