动物造模 z-bio 2023-04-18 488

　　Objective: To study the genotoxicity of aniline and its repair kinetics.

　　Methods: Single-cell gel electrophoresis (SCGE) technology was used to detect the relationship between DNA damage and time-course of hepatocytes and lymphocytes in KM mice after a single gavage of 100 mg/kg aniline for 3, 8, 16, 24, and 32 hours.

　　Results: The results of SCGE experiment showed that the tail length and tail moment of hepatocytes gradually increased from the beginning of 8 h, and reached the maximum level of DNA damage at 16 h. Compared with the control group, the difference was significant (P<0.01). 32="" the="" level="" of="" dna="" damage="" gradually="" at="" h="" p="">0.05); while the tail length and tail moment of lymphocytes gradually increased at the beginning of 16 h, reached the maximum at 24 h, and the DNA damage gradually recovered at 32 h .

　　Conclusion: Aniline has potential genotoxicity to hepatocytes and lymphocytes; the changes of the two DNA damage indicators have a significant time effect relationship, indicating that these two cells have an effective DNA repair mechanism.