动物造模 z-bio 2022-12-12 727

　　Objective: To discuss the effect of hesperidin (HDN) on the antioxidant effect of the body.

　　Methods: Spectrophotometry, pyrogallol auto-oxidation method and Fe2+-phenanthroline method were used to detect HDN in vitro to remove free radicals, inhibit mitochondrial swelling and red blood cell oxidative hemolysis; experimental mice were fed with different concentrations of HDN (0, 80, 160, 320 mg/kg) for 12 consecutive days, ELISA and spectrophotometry were used to detect the content of MDA and the vitality of antioxidant enzymes (SOD, CAT, GSH-PX) in mouse tissues, and RT-PCR technology to analyze the expression level of antioxidant enzymes mRNA .

　　Results: Compared with the control group, the removal rate of free radicals (·OH, O2-·, DPPH·) in HDN group was significantly improved, and the oxidative hemolysis and mitochondrial swelling of mouse erythrocytes were significantly reduced in vitro; The vitality of oxidase SOD, CAT and GSH-PX was significantly higher than that of the control group, and the expression of antioxidant enzyme mRNA (SOD, CAT, GSH-PX) in the tissue was up-regulated.

　　Conclusion: HDN can eliminate free radicals, reduce the oxidative damage of cells caused by free radicals, inhibit the generation of peroxides, up-regulate the expression of antioxidant enzymes and improve the vitality of enzymes, showing a good antioxidant effect.