动物造模 z-bio 2023-04-27 540

　　OBJECTIVE: To discuss the effects of telemetry EEG activity in the sublimbic region on drug-seeking behavior in morphine-dependent rats during drug-seeking decline and its mechanism.

　　Methods: The SD rats were randomly divided into the model group and the control group, and the brain plane positioning electrodes were implanted in the IL area. The rats in the model group were made into morphine dependence and recession models in turn, and the wireless telemetry system was used to record the brain of the IL area of the rats during the recession period. Changes in electrical activity.

　　Results: The white box residence time of the rats in the model group was significantly better than that before morphine injection and the control group for 1-2 days after withdrawal; 1-2 days after the decline exercise, the white box residence time of the rats in the model group was significantly shorter than the withdrawal period, which was similar to that of morphine. There was no significant difference between pre-injection and control group. Compared with the control group, when the model group stayed in the white box for 2 days of withdrawal, the EEG β wave in the IL area increased significantly, and the δ wave decreased significantly; when passing from the black box to the white box, the δ wave increased significantly, and the α wave and β wave decreased significantly. . After 2 days of decline, when the rats in the model group stayed in the white box, the EEG in the IL region decreased significantly compared with the control group, the β wave and theta wave significantly decreased, and the δ wave increased significantly compared with the withdrawal period; , compared with the control group, the IL EEG had no significant difference, but compared with the withdrawal period, the delta wave was significantly reduced, and the alpha and beta waves were significantly increased.

　　Conclusion: Morphine-dependent rats have abnormal changes in the brain electrical activity in the IL region during the drug-seeking decline period. The generation of this abnormal EEG may affect the composition of drug-seeking motivation, thereby inhibiting the expression and maintenance of drug-seeking behavior.