动物模型,NIH裸小鼠感染 z-bo 2020-08-11 543

　　(1) Replication method Take 4-week-old NIH nude mice and inoculate intraperitoneally with 100TCID10 to the -4 power/0.5ml virus 0.2ml, the strain is EHFV LN-84L. Virus examination: virus antigens were detected in the cytoplasm of lung epithelial cells, capillary endothelial cells, cerebral cortical nerve cells, renal tubular epithelial cells, cardiomyocytes, and some liver cells. The virus is mainly distributed in lung, brain, and kidney, followed by heart and liver, and virus antigens are mainly distributed in the cytoplasm of corresponding tissue cells. Electron microscopy: a large number of Bunia-like virus particles and viral inclusion bodies were found in brain, lung, kidney and other tissues.

　　(2) Model characteristics: The brain, heart, liver, spleen, lung, and kidney of model animals all have specific antigens, and the antigen fluorescence in the brain is the brightest. Judging from the onset symptoms of this model animal, there are not only symptoms of the digestive system, but also capillary damage and pathological changes of the nervous system, indicating that EHFV's invasion of the organs and tissues of nude mice is broadly tropic.

　　(3) Comparative medicine. The incubation period of nude mice after infection with the virus is 7-12 days, and clinical symptoms and signs appear from 8-13 days. They start to be listless, less active, slow in response, weight loss, and skin thinning; then animals lose weight more Obviously, he lost weight sharply, had difficulty moving, and often hugged him together. When he was dying, his limbs were paralyzed. The onset cycle of animals is short, from onset to death, the average is about 1 day, suitable for clinical research on acute infection of HF. HF infected nude mice of different age groups (maximum 2 months old) and all of them died of the disease, while the immune-sound mice only died within 14 days of age. It indicates that cellular immunity may play a major role in anti-HV infection. This model confirms that nude mice are ideal model animals for HV infection.