动物造模,黑色素瘤 z-bio 2022-02-09 436

　　Objective: To investigate the effect of high-intensity focused ultrasound (HIFU) treatment on the in vivo metastasis of melanoma cells in a mouse model of melanoma.

　　METHODS: Mouse melanoma cells B16-F10 were injected subcutaneously to construct a mouse melanoma subcutaneous transplanted tumor model; when the longest diameter of the tumor reached 7-10 mm, HIFU treatment was performed, and an experimental control group was set up (tumor-bearing mice irradiated HIFU but keep the power source switch of the treatment head turned off, namely the sham group); the long and short diameters of the tumor were measured every 3 d after treatment for a total of 3 weeks, the tumor curve was drawn, the survival rate and metastasis rate were calculated, and real-time fluorescence quantitative The relative expression levels of 3 markers of melanoma cells in blood were determined by PCR to monitor the number of circulating melanoma cells in blood, which are melanoma-associated antigen A3 (MAGE-A3), melanoma antigen recognized by T cell 1 (MART1 ) and homologous transformation into box gene transcription factor PAX3); 14 days after HIFU treatment, B16-F10 cells were re-transplanted by tail vein injection, and the lung metastasis rate was observed.

　　Results: (1) The respective median survival time and 95 % confidence interval (CI): the sham group was 19.00 d and 17.14-20.86 d, respectively, and the HIFU group was 26.00 d and 24.76-27.25 d, respectively. The survival rate was different. show

　　From the 20th day after treatment, the tumor volume of the two groups of mice was significantly different, and from the 20th day after HIFU treatment, including the 23rd and 26th day after treatment, the tumor volume of the mice in the irradiation group It was significantly smaller than the sham control group (P<0.05); (2) The real-time fluorescence quantitative PCR results showed that the three indexes of MAGE-A3, MART1 and PAX3 in the HIFU group were significantly lower than those in the sham control group on the 7th day after treatment (P <0.05), the expression of MAGE-A3 was still significantly lower than that of the sham control group on the 14th day after treatment (P<0.05); (3) The same tumor cells were re-transplanted through the tail vein 14 days after HIFU treatment, the HIFU group The number of lung metastases was significantly lower than that in the sham control group (P<0.05), and the tumor metastasis inhibition rate was 42.4%.

　　Conclusion: HIFU treatment can inhibit the metastasis of melanoma cells in vivo, and the mechanism may involve reducing the shedding of tumor cells from the primary tumor and the colonization ability of tumor cells in the lung.