动物造模,帕金森病模型 z-bio 2022-07-01 585

　　Objective To investigate the neuroprotective effect of the total flavonoids of Mongolian medicine Xiangqinglan on MPTP-induced Parkinson's model mice.

　　Methods The MPTP-induced Parkinson's disease model was established, and the effects of TFDM on the motor function of PD mice were evaluated by rotating rod test and rod climbing test; MDA), glutathione peroxidase (GSH-Px) expression; by immunohistochemistry and Western blot detection of TFDM on tyrosine hydroxylase (tyrosine hydroxylase, TH) and apoptosis-related protein Caspase-3 , Bcl-2, Bax expression.

　　Results The results of behavioral evaluation showed that TFDM treatment significantly increased the fall latency of mice (P<0.05), and significantly decreased the climbing time of mice (P<0.05); ELISA results showed that TFDM treatment significantly reduced the MDA level At the same time, the expressions of SOD and GSH-Px were significantly increased (P<0.05); the results of immunohistochemistry and Western bolt showed that the TH, Bcl-2, Bcl- 2/ The expression level of Bax protein was significantly increased (P<0.05), while the expression levels of Caspase-3 and Bax were significantly decreased (P<0.05).

　　Conclusion TFDM can improve motor dysfunction, reduce oxidative stress injury and reduce the loss of dopaminergic neurons in PD model mice, and may play a neurological role by inhibiting the activity of Caspase-3 protein, up-regulating Bcl-2 and down-regulating the expression of Bax protein. Protective effects.