动物造模,高血脂 z-bio 2022-02-10 307

　　Objective To investigate the effect of Xiaoxianxiong Decoction on vascular endothelial injury in hyperlipidemia mice.

　　Methods Thirty-six C57BL/6 mice were selected and randomly divided into control group, model group, low-dose group (0.03 g/mL Xiaoxianxiong Decoction), and middle-dose group (0.06 g/mL Xiaoxianxiong Decoction). , high-dose group (0.12 g/mL Xiaoxianxiong decoction) and lovastatin group (2.5 mg/kg lovastatin), 6 animals in each group, fed with high-fat diet for 8 weeks to establish HLP model and intervene with appropriate drugs. During the administration period, the general behavioral status of the mice in each group was observed, and the mice were weighed once a week; HE staining was used to observe the pathological changes of aortic blood vessels; immunohistochemical method was used to detect the von Willebrand factor (von Willebrand factor) in the aortic tissue of mice. qRT-PCR detection of mouse aortic tissue vascular endothelial growth factor (VEGF), tyrosine protein kinase Eph receptor B4 (EPH receptor B4, EphB4) and its membrane adhesion The mRNA level of EphrinB2 (EphrinB2); Western blot was used to detect the protein levels of VEGF, EphB4 and EphrinB2 in mouse aortic tissue.

　　Results Compared with the control group, the mice in the model group had decreased activity and food intake, poorer hair, increased body weight, and increased expression levels of vWF, VEGF, EphB4, and EphrinB2 in aortic tissue; The activity and food intake of mice in each dose group and lovastatin group increased, their hair was shiny, their body weight decreased, and the expression levels of vWF, VEGF, EphB4, and EphrinB2 in aortic tissue decreased (P<0.05); Compared with other groups, the mRNA and protein expression levels of VEGF, EphB4 and EphrinB2 in the aortic tissue of mice in the middle and high dose groups were decreased (P<0.05).

　　Conclusion Xiaoxianxiong Decoction can inhibit the development of HLP and has a protective effect on the vascular endothelium of mice.