动物造模,药物性肝损伤 z-bio 2022-06-02 545

　　Objective Paijiangcao is the whole rooted herb of the perennial herbs of the family Paiaceae, Huanghuabaijiang and Baihuabaijiang, with various pharmacological activities.

　　Methods The rats were randomly divided into 6 groups by tail vein injection of acetaminophen: control group, model group, low-dose Patrinia spp. . Simultaneously with the modeling, the low, medium and high dose groups of Patrinia spp. were given 4, 8, and 16 g/kg of Patrinia spp. by gavage every day. The model group was given an equal volume of normal saline by gavage for 30 consecutive days. Liver index was calculated; HE staining was used to detect the degree of liver tissue pathological damage; kits were used to detect the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in serum; enzyme-linked The levels of interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and interleukin 1β (IL-1β) were detected by immunosorbent assay; total superoxide dismutase (SOD), total superoxide dismutase (SOD), total superoxide dismutase (SOD), Malondialdehyde (MDA), glutathione peroxidase (GSH-Px) content.

　　Results Compared with the control group, the liver index of the model group was significantly increased (P<0.05), the levels of AST, ALT, and ALP were significantly increased (P<0.05), and the levels of TNF-α, IL-6, and IL-1β were significantly increased. (P<0.05), the contents of SOD and GSH-Px were significantly decreased (P<0.05), and the contents of MDA were significantly increased (P<0.05). Compared with the model group, the liver index of the medium and high dose groups and the bifendate group were significantly decreased ( P<0.05), the degree of pathological damage was significantly improved, and the levels of AST, ALT and ALP were significantly decreased (P<0.05). The levels of TNF-α, IL-6 and IL-1β were significantly decreased (P<0.05), the contents of SOD and GSH-Px were significantly increased (P<0.05), and the content of MDA was significantly decreased (P<0.05).

　　Conclusions Paijiangcao has a protective effect on acetaminophen-induced drug-induced liver injury in rats by improving the pathological damage of liver tissue, inhibiting the release of inflammatory factors, and improving oxidative stress.