动物造模,肺组织损伤 z-bio 2022-04-27 394

　　Objective To evaluate the effects of three kinds of dental grinding dust, cobalt chromium molybdenum metal, zirconium dioxide porcelain and polymethyl methacrylate resin, on lung tissue damage in rats.

　　Methods Scanning electron microscope and laser particle size analyzer were used to detect the surface morphology and particle size of three kinds of dental dust, and a rat dust-exposed animal model was established; Giemsa staining and enzyme-linked immunosorbent assay were used to detect rat bronchoalveolar lavage The total number of leukocytes, the number of macrophages and the contents of interleukin-6 and interleukin-16 in the fluid were measured; the pathological changes of lung tissue sections were observed by HE staining.

　　Results The surface morphology and particle size distribution of the three types of dental grinding dust particles were different, and the particle size of the dust was significantly smaller after fine grinding; compared with the control group, the number of leukocytes, macrophages and interleukin-6 The contents of IL-16 and IL-16 were significantly increased, the inflammatory cell infiltration in the lung tissue of the rats was obvious, and the structure of the lung tissue was destroyed and fibrotic; compared with the non-fine grinding group, the inflammatory cells and Inflammatory factors were significantly increased.

　　Conclusion The three kinds of dental dusts before and after grinding can cause inflammatory damage and early fibrotic changes in the lung tissue of dust-exposed rats. Damage increases.